Description the rc dc reducing agent and detergent compatible protein assay is a colorimetric assay for protein determination in the presence of reducing agents and detergents. B, typical standard curve for bradfordbased assays, including bio rad protein assay and quick start bradford protein assay. The dc protein assay kit by bio rad is used for the detection and quantification of protein following detergent solubilization. The detection is via a colorimetric assay similar to the lowry assay, whereby a twostep reaction yields a color change that occurs in the presence of amide bonds. Modified bio rad protein assay 30 rc dc protein assay 31 chapter 10 firstdimension separation methods 32 protein load for 2d gels 32 ipg strip rehydration 32 passive rehydration with sample 32 active rehydration 33 performing ief 33 positioning strips and use of wicks 33 focusing conditions for ipg strips on the protean ief cell 34. They adsorb less coomassie blue dye than glass cuvettes and much less than quartz cuvettes. Bio rad house 8687, udyog vihar phase iv gurgaon 122005 haryana. The bio rad dc protein assay is a colorimetric assay for protein concen tration following detergent solubilization. The rc dc assay is based on the principle of lowry estimation, where protein reacts with alkaline copper and subsequently reduces the folin. Jul 17, 2007 smaller range of protein detection than with most assays e. Rcdc protein assay from biorad protein estimation is one of the most basic and crucial experiments in biochemistry.
Attenuation of metmediated migration and invasion in hepatocellular carcinoma cells by socs1 article snippet. The bio rad dc protein assay is a modified lowry assay. When performing a protein assay using any of the suggested protein assay systems, an equal concentration of lysis buffer or reagent must be included in the standard curve for optimal accuracy. Do you have experience in protein quantitation using the rcdc. Quick start bradford protein assay kits from biorad. Bio rad offers four colorimetric assays for protein quantitation. Biorad rc dc protein assay the rc dc protein assay is a colorimetric assay for protein quantification with all the functionality of the original dc protein assay.
The rc dc protein assay is a colorimetric assay for protein quantitation with all the functionality of the original dc protein assay. Bio rad modified the widely used lowry method lowry et al. For additional information regarding the lowry assay and reagents, please refer to the manufacturers product literature supplied with the dc protein assay. The bio rad assay has several improvements over the original assay in that colour development is.
The rc dc reducing agent and detergent compatible protein assay is a colorimetric assay for protein determination in the presence of reducing agents and. Compatibility of pierce bca protein assay with promega lysis. Rc dc protein assay instruction manual catalog biorad. Rc dc protein assay kit ii, supplied by bio rad, used in various techniques. This assay, based on the dc protein assay, is a modified form of the lowry assay that is both reducing agent compatible rc and detergent compatible dc, whereas the dc assay is only detergent compatible. Rcdc kit from biorad is generally used to quantify the protein when it is having any detergent or reducing agent or both. Protein quantitation assays frequently asked questions. The reaction is similar to the welldocumented lowry1 assay, but with the following improvements. Rc dc protein quantification assay, supplied by bio rad, used in various techniques. Bio rad rc dc protein assay the rc dc protein assay is a colorimetric assay for protein quantification with all the functionality of the original dc protein assay. The bio rad dc protein assay is a colorimetric assay for protein concentration following detergent solubilization. Each assay was performed in a microplate using bsa standards spiked with detergent or water control, and followed the manufacturers instructions. Bradford assay bradford reagent thermo fisher scientific kr. The reaction is similar to the welldocumented lowry assay, but has been modified to save time.
We providing 1 pdf manuals bio rad dc protein assay for download free by document types. The concentrations represent maximum concentrations for standard assay. Rc dc protein assay, supplied by bio rad, used in various techniques. The dc protein assay requires only a single 15 minute incubation, and absorbance is stable for at least 2 hours. The protein assay can be performed in either test tube or microplate format. A e n f native ief 1d sds page sale prep chromatography liuid ief n c where to use a protein assay 3, compatible. Try the rc dc protein assay for rapid protein determination in the presence of reducing agents andor detergents. The best part of this kit is revealed in section 1, page 1 of the user manual. Protein assays reagent compatibility chart for biorad. Blood protocols alt assay bun assay ldh assay plasma dna preparation plasma preparation. The colloidal gold assay and dc protein assay are both independently effective in analysis of surface protein concentration in protein colloids. Similar to the lowry assay, but with the following improvements. Use the rc dc protein assay for samples containing reducing agents and detergents. The reaction reaches 90% of its maximum color development within 15 minutes thereby.
Bradford assay bradford reagent thermo fisher scientific jp. Owners manuals and users guides for measuring instruments bio rad rc dc protein assay. Bio rad protein assay, dc protein assay, and rc dc protein assay. Without cumbersome sample preparation or buffer exchange steps, protein concentration can be determined using one simple protocol. Quick start bradford protein assay bio rad protein. Dc protein assay kits, supplied by bio rad, used in various techniques. The rc dcprotein assay is a colorimetric assay for protein quantitation with all the functionality of the original dc protein assay. Bio rad rc dc protein assay cytochrome c elisa immunoprecipitation protein carbonylation in test tube protein carbonylation on membrane tissue fractionation by differential centrifugation. The standard working range is 1001500 gml with up to 1% detergent or higher in some cases. Bio rad disposable cuvettes are molded from polystyrene, making them ideal for use with the bio rad protein assay, the dc protein assay, and the rc dc protein assay.
This assay is based upon a simple reaction whereby the protein within a solution reacts with a folin reagent and an alkaline copper tartate solution. Determine protein concentration by plotting the absorbance vs. The dc protein assay is a modified lowry assay which works in the presence of. This assay is based on the lowry1 assay but has been modified to be reducing agent compatible rc as well as detergent compatible dc. The dc protein assay is one of many colormetric assays commercially available for protein concentration quantification. This assay, based on the dc protein assay, is a modified form of the. The dc detergent compatible protein assay is a colorimetric assay for protein concentration following detergent solubilization. We providing 1 pdf manuals bio rad rc dc protein assay for download free by document types. After the protein concentration was determined by the bio rad protein assay, 40 g of cell lysates from each group was used to analyze the expression of macrophagesignaling proteins via the cst signalpathway chip cell signaling technology, inc. Compatible with detergents, reducing agents, laemmli sample buffer with 5% betamercaptoethanol, etc see list of compatible reagents on biorad cataloge minimum incubation time 15minutes.
The rc dc protein assay is a colorimetric assay for protein quantification with all the functionality of the original dc protein assay. Jul 31, 2007 the bio rad dc detergent compatible assay is a colorimetric method for measuring protein concentrations. The bottom line bio rad s quick start kit provides the standards and detection solutions necessary to rapidly and reliably measure protein concentrations at different volumes and over a wide range of protein concentrations. Accurate estimation is necessary for assessing the levels of specific protein for western blotting which has a sensitivity range from nano to picograms of protein. Bradford assay bradford reagent thermo fisher scientific hk. The rc dc protein assay kit provides all necessary reagents for 500 standard assays or 2,000. Following centrifugation at 15000 g for 20 min, the supernatant was collected and protein concentration determined using the rc dc protein assay kit bio rad, mississauga, on, united states. The rc dc protein assay has all the unique features of the original dc protein assay, but covers a broader range of reagents. Bio rad laboratories nv winninglaan 3 be9140 temse office.
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